Chromatography is a family of separation methods used in peptide quality control. Reversed-phase HPLC with UV detection is the most common method for quantitative purity; mass spectrometry is often used as an orthogonal identity technique. The choice of column, eluent, gradient, and integration rules all affect the reported “% purity” on a COA, so method comparability is essential when re-testing a batch in-house.
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