Short answer: Enter lyophilized vial mass (mg), diluent volume (mL), and a target aliquot mass (mcg) from published research literature to calculate stock solution concentration (mcg/mL) and aliquot volume. Laboratory preparation math only — not for human or veterinary use.
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Accurate stock solution preparation is critical for reproducible preclinical and analytical research. This tool handles standard dilution math: concentration from total mass and volume, and aliquot volume from a target mass at that concentration.
Concentration (mcg/mL) = (mg × 1,000) ÷ mL
Aliquot volume (mL) = target mcg ÷ concentration
5 mg + 2 mL: 5,000 mcg ÷ 2 mL = 2,500 mcg/mL.
10 mg + 2 mL: 10,000 mcg ÷ 2 mL = 5,000 mcg/mL.
30 mg + 3 mL: 30,000 mcg ÷ 3 mL = 10,000 mcg/mL.
For reconstitution technique in research settings, see how to reconstitute peptides and how to read a peptide COA. For vendor comparison before procurement, see our scoring methodology.
Calculator output is only as accurate as the mass on the vial. Match label and COA before preparation.
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Enter total lyophilized peptide mass in milligrams (mg), diluent volume in milliliters (mL), and a target aliquot mass in micrograms (mcg) from published research literature. The tool outputs stock solution concentration in mcg/mL and the aliquot volume required. For laboratory preparation math only — not personal use guidance.